Ultra scale-down tools have demonstrated the huge benefit for rapid process development with reduced material requirement and better solutions. However, use of stirred cell devices for process optimisation have been primarily limited due to its geometric and flow dissimilarity to crossflow filtration (CFF) at scale, and thus actual process optimisation runs are usually done using CFF cassettes, which tend to be linearly scalable but their use are limited by time and material constraints at early stages in process development.
Computational fluid dynamics (CFD) is a tool that is primarily used to further understanding of fluid flow in complex geometries, and has been widely used in aeronautics, structural mechanics, heat transfer, fluid flow, chromatography and more recently, flow optimisation in screened CFF channels. COMSOL Multiphysics® was used to model V- and C-screen Pellicon 2 cassettes and the USD shear membrane device to develop wall shear rate correlations for both, and scale-up carried out using equivalent averaged wall shear rates as the scaling parameter.
Rules of scale-up were looked at and a flux prediction protocol was developed to predict TFF performance at scale using CFD techniques and USD data. Escherichia coli homogenate and Saccharomyces cerevisiae was used as feed material for clarification/recovery of a 46 kDa antibody fragment and primary recovery case studies respectively. Scale-up was successfully carried out using...
Session: M3 - Cross Flow Techniques
Day: 14 March 2018
Time: 13:00 - 14:15 h